5H07
TNIP2-Ub complex, C2 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2015-07-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.765, 80.972, 65.352 |
| Unit cell angles | 90.00, 114.28, 90.00 |
Refinement procedure
| Resolution | 19.858 - 2.586 |
| R-factor | 0.2177 |
| Rwork | 0.211 |
| R-free | 0.27340 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.586 |
| Number of reflections | 12803 |
| <I/σ(I)> | 13.4 |
| Completeness [%] | 99.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | ammonium acetate, PEG3350, Tris-HCl |
