5GZV
Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.989, 127.229, 161.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.610 |
| R-factor | 0.20514 |
| Rwork | 0.203 |
| R-free | 0.25343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 70506 | |
| <I/σ(I)> | 41.44 | 6.08 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | (NH4)2HPO4, Na-citrate |
