5GZB
Crystal Structure of Transcription Factor TEAD4 in Complex with M-CAT DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 118.595, 118.595, 110.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.500 - 2.704 |
| R-factor | 0.2318 |
| Rwork | 0.229 |
| R-free | 0.25620 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 20397 | |
| <I/σ(I)> | 20.3 | 1.5 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 9.3 | 9.1 |
| CC(1/2) | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.6 M Na2HPO4, 0.6 M KH2PO4, 0.1 M HEPES |
