5GZ0
Crystal structure of FM329, a recombinant Fab adopted from cetuximab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.521, 68.031, 97.780 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 47.850 - 1.700 |
| R-factor | 0.1885 |
| Rwork | 0.188 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yy8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.082 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Number of reflections | 101903 | |
| <I/σ(I)> | 28.08 | 3.14 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 20% (w/v) PEG 3000, 100 mM Imidazole, Hydrochloric acid pH 8.0, 200 mM Zinc acetate |
