5GYG

Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	42.253, 70.886, 85.254
Unit cell angles	90.00, 90.23, 90.00

Refinement procedure

Resolution	50.000 - 1.740
R-factor	0.1768
Rwork	0.174
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gv1
RMSD bond length	0.011
RMSD bond angle	1.379
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	1.800
High resolution limit [Å]	1.740	3.750	1.740
Rmerge	0.032	0.025	0.095
Number of reflections	48098
<I/σ(I)>	22.9
Completeness [%]	93.2	89.1	82.1
Redundancy	2.1	2.2	1.8
CC(1/2)		0.998	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		298	PEG 3350, 0.1 M tri-sodium acetate pH 4.5, 0.1 M Bis-Tris pH 5.5