5GY6

Ribonuclease from Hericium erinaceus (RNase He1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	293
Detector technology	CCD
Collection date	2016-03-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	31.701, 42.945, 60.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.958 - 1.500
R-factor	0.1736
Rwork	0.172
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3who
RMSD bond length	0.011
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.960
High resolution limit [Å]	1.500
Number of reflections	13702
<I/σ(I)>	12.31
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	31% PEG-MME 550, 0.1M MES pH 7.0, 5mM ZnSO4