5GXG
High-resolution crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-24 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.490, 77.956, 59.968 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 59.660 - 1.700 |
R-factor | 0.15788 |
Rwork | 0.156 |
R-free | 0.19158 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w9c |
RMSD bond length | 0.020 |
RMSD bond angle | 2.137 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.660 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.084 | |
Number of reflections | 51169 | |
<I/σ(I)> | 39.2 | |
Completeness [%] | 99.6 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M HEPES, 0.1M Sodium Chloride, 1.6M Ammonium sulfate |