5GXB
crystal structure of a LacY/Nanobody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 96.543, 96.543, 145.025 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.342 - 3.300 |
| R-factor | 0.34 |
| Rwork | 0.340 |
| R-free | 0.34650 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.844 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((dev_2405: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.342 | 3.600 |
| High resolution limit [Å] | 3.300 | 3.500 |
| Number of reflections | 10364 | |
| <I/σ(I)> | 7.9 | 1.5 |
| Completeness [%] | 97.1 | 96.4 |
| Redundancy | 29.2 | 15.3 |
| CC(1/2) | 0.988 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100mM MES pH 6.0, 100mM (NH4)2C4H4O6, 43% PEG 400 |
