5GX9
PYP mutant - E46Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | NUCLEAR REACTOR |
| Source details | JRR-3M BEAMLINE 1G-B |
| Synchrotron site | JRR-3M |
| Beamline | 1G-B |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 2.6 |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.823, 66.823, 40.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.445 - 1.493 |
| R-factor | 0.1623 |
| Rwork | 0.158 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ota |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Refinement software | PHENIX ((1.12rc1_2815)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.550 |
| High resolution limit [Å] | 1.493 | 1.493 |
| Rmerge | 0.095 | 0.318 |
| Number of reflections | 15995 | |
| <I/σ(I)> | 7.78 | 2.4 |
| Completeness [%] | 93.5 | 85.7 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | AMMONIUM SULPHATE, SODIUM CHLORIDE, SODIUM DIDEUTERIUM PHOSPHATE, DISODIUM DEUTERIUM PHOSPHATE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, MICROSEEDING, temperature 295K |
