5GWN
Crystal structure of human RCC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.447, 84.056, 60.671 |
| Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
| Resolution | 40.676 - 1.309 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.18890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a12 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.084 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.676 | 1.360 |
| High resolution limit [Å] | 1.309 | 1.309 |
| Number of reflections | 109282 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 98.1 | 96.6 |
| Redundancy | 12.8 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M BIS-TRIS 5.5, 0.2M (NH4)2SO4, 16% PEG 3350 |
