5GWF
FraC with GlcNAc(6S) bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.160, 44.400, 114.360 |
| Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
| Resolution | 37.060 - 1.550 |
| R-factor | 0.11895 |
| Rwork | 0.118 |
| R-free | 0.16307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.529 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.060 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.069 | 0.290 |
| Number of reflections | 103405 | |
| <I/σ(I)> | 14.8 | 4.5 |
| Completeness [%] | 91.8 | 76.8 |
| Redundancy | 5.3 | 4.8 |
| CC(1/2) | 0.997 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293.15 | 18% PEG8000, 210mM ammonium sulfate, 100mM sodium cacodylate |
