5GWD
Structure of Myroilysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2816 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.223, 35.486, 93.757 |
| Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.890 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.847 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.128 | 0.701 |
| Number of reflections | 37660 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 98.6 | 91 |
| Redundancy | 10.6 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Sodium Citrate |
