5GW0

Crystal structure of SNX16 PX-Coiled coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	70.380, 196.780, 72.960
Unit cell angles	90.00, 111.75, 90.00

Refinement procedure

Resolution	19.991 - 3.300
R-factor	0.239
Rwork	0.236
R-free	0.28800
RMSD bond length	0.009
RMSD bond angle	1.381
Data reduction software	Aimless
Data scaling software	Aimless (0.5.12)
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.770	56.770	3.500
High resolution limit [Å]	3.300	9.900	3.300
Rmerge	0.087	0.056	0.633
Rmeas	0.102	0.066	0.741
Rpim	0.052	0.034	0.383
Total number of observations	98589	3117	16305
Number of reflections	27200
<I/σ(I)>	10.6	33	2.1
Completeness [%]	98.3	83.4	99.5
Redundancy	3.6	3.6	3.6
CC(1/2)	0.996	0.993	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	31mM Citric, 69mM Bis-tris Propanol, 18% PEG 5000, 70mM NaF, 2mM TCEP