5GVJ

Structure of FabK (M276A) mutant from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	84.096, 115.129, 67.913
Unit cell angles	90.00, 106.59, 90.00

Refinement procedure

Resolution	34.649 - 1.900
R-factor	0.1817
Rwork	0.181
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gvh
RMSD bond length	0.007
RMSD bond angle	0.829
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	48755
<I/σ(I)>	39.5
Completeness [%]	96.3
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM DTT 1 mM FMN