5GVC
Human Topoisomerase IIIb topo domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2014-05-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.088, 93.665, 91.608 |
| Unit cell angles | 90.00, 91.88, 90.00 |
Refinement procedure
| Resolution | 49.718 - 2.436 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cgy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.685 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 55090 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES-Na, 0.2 M MgCl2, 25% PEG3350 |
