5GV4
Y74COX MUTANT OF PLASMODIUM FALCIPARUM TRIOSEPHOSPHATE ISOMERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-07-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.620, 65.620, 141.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 2.090 |
R-factor | 0.19979 |
Rwork | 0.198 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vfh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.254 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.140 | 2.120 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.100 | 0.410 |
Number of reflections | 19232 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 97.2 | 64.4 |
Redundancy | 9.3 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | MES 100 mM, PEG 8K 18%, EDTA (10 mM), CaCl2 (10 mM), Sodium Azide 0.006%, 10% Ethylene glycol, Phosphoglycolic acid (50x molar excess) |