5GUH

Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	61.899, 114.619, 136.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.835 - 2.400
R-factor	0.2106
Rwork	0.209
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4olb
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.620	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.121
Number of reflections	38755
<I/σ(I)>	8
Completeness [%]	99.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50mM potassium phosphate monobasic, 20% PEG8000