5GUF

Structural insight into an intramembrane enzyme for archaeal membrane lipids biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	300
Detector technology	CCD
Collection date	2016-04-23
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9973
Spacegroup name	C 2 2 21
Unit cell lengths	56.505, 89.249, 98.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.625 - 2.397
R-factor	0.2114
Rwork	0.207
R-free	0.24890
RMSD bond length	0.020
RMSD bond angle	0.994
Data reduction software	Aimless
Refinement software	PHENIX ((dev_2719))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.700
High resolution limit [Å]	2.380
Number of reflections	9961
<I/σ(I)>	24.4
Completeness [%]	98.5
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	200mM NaCl, 20% (w/v) PEG 400, 100mM Tris/HCl pH 8.0