5GU5

Crystal structure of p24gamma2 GOLD domain determined by sulfur-SAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2013-06-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 64 2 2
Unit cell lengths	79.410, 79.410, 125.948
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.833 - 2.800
R-factor	0.2261
Rwork	0.224
R-free	0.27390
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.10_2155: 000))
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge		0.762
Number of reflections	6265
<I/σ(I)>	72.1	4.5
Completeness [%]	99.0	100
Redundancy	12.8	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	60% (v/v) Tascimate (pH 8.0)