5GTU
Structural and mechanistic insights into regulation of the retromer coat by TBC1d5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.881, 63.832, 78.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.500 |
| R-factor | 0.14089 |
| Rwork | 0.139 |
| R-free | 0.18555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2x |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.288 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 33729 |
| <I/σ(I)> | 38 |
| Completeness [%] | 98.1 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 289 | 0.1M Bis-Tris, pH6.0, 2% Tacsimate, pH 6.0, 15~20% PEG3350 |
