5GTB

crystal structure of intermembrane space region of the ARC6-PDV2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	H 3 2
Unit cell lengths	151.228, 151.228, 100.566
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.438 - 2.871
R-factor	0.267
Rwork	0.265
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5had
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.438	2.920
High resolution limit [Å]	2.871	2.870
Rmerge	0.076
Number of reflections	10239
<I/σ(I)>	33.8
Completeness [%]	93.8
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG2000MME, Tris pH6.5