5GSZ
Crystal Structure of the KIF19A Motor Domain Complexed with Mg-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 122.604, 122.604, 56.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.320 - 2.720 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.132 | 0.995 |
| Number of reflections | 12043 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 13.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Ethylene Glycol, PEG8000, Tris-Bicine, Magnesium chloride, Calcium chloride |
