5GSQ

Crystal structure of IgG Fc with a homogeneous glycoform and Antibody-Dependent Cellular Cytotoxicity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	50.183, 158.406, 66.905
Unit cell angles	90.00, 109.04, 90.00

Refinement procedure

Resolution	49.421 - 1.850
R-factor	0.229
Rwork	0.227
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3do3
RMSD bond length	0.011
RMSD bond angle	1.308
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.850	3.993	1.850
Rmerge	0.037	0.021	0.787
Number of reflections	78598
<I/σ(I)>	15.2
Completeness [%]	93.8	89	91.4
Redundancy	2.8	2.6	2.6
CC(1/2)		0.997	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1M sodium acetate trihydrate, pH4.5, 30% (w/v) PEG 3350