5GS6

Full-length NS1 structure of Zika virus from 2015 Brazil strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97853
Spacegroup name	P 41 2 2
Unit cell lengths	96.948, 96.948, 270.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.474 - 2.852
R-factor	0.2457
Rwork	0.244
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o6b
RMSD bond length	0.004
RMSD bond angle	0.937
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	30984
<I/σ(I)>	24.152
Completeness [%]	100.0
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M Tris hydrochloride pH 8.5, 2.0M ammonium sulfate