5GS0

Crystal structure of the complex of TLR3 and bi-specific diabody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	98.519, 141.329, 150.831
Unit cell angles	90.00, 106.88, 90.00

Refinement procedure

Resolution	35.074 - 3.275
R-factor	0.1945
Rwork	0.192
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ulu 5gru
RMSD bond length	0.011
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.275	3.300
Number of reflections	58328
<I/σ(I)>	18.5
Completeness [%]	96.6
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	296	0.1 M Sodium citrate tribasic pH 5.5, 19.5% PEG 2,000, 0.75 M Sodium chloride