5GQQ

Structure of ALG-2/HEBP2 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-09
Detector	RAYONIX MX-225
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	47.279, 78.124, 87.171
Unit cell angles	113.71, 105.64, 93.95

Refinement procedure

Resolution	25.935 - 2.200
R-factor	0.1958
Rwork	0.194
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.208
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
<I/σ(I)>	18.78
Completeness [%]	94.9
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	0.1 M Bis-Tris, 2 M NaCl, pH 5.5