5GQO

Structure of the second Single Stranded DNA Binding protein (SSBb) from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5419
Spacegroup name	P 65 2 2
Unit cell lengths	73.610, 73.610, 216.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.240 - 2.500
R-factor	0.21334
Rwork	0.211
R-free	0.25627
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eiv
RMSD bond length	0.016
RMSD bond angle	1.775
Data reduction software	iMOSFLM (7.0.9)
Data scaling software	SCALA
Phasing software	PHASER (2.5.5)
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.240	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.093	0.100
Number of reflections	12839
<I/σ(I)>	16.4	2.3
Completeness [%]	99.9	100
Redundancy	9.9	9.6
CC(1/2)	0.999	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	0.2M calcium chloride dihydrate, 0.1M HEPES pH 7.5, 7.5 % (v/v) PEG 400, 3% (w/v) 1,5-diaminopentane dihydrochloride