5GQ1
Crystal structure of 2C helicase from enterovirus 71 (EV71)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.109, 159.654, 75.606 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 43.635 - 2.493 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.052 | 0.621 |
| Number of reflections | 84048 | |
| <I/σ(I)> | 13.32 | 1.67 |
| Completeness [%] | 98.7 | 94.7 |
| Redundancy | 3.38 | 3.07 |
| CC(1/2) | 0.998 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES, 10% PEG6000, 5% MPD and 0.5% w/v polyvinylpyrrolidone K15 |
