5GPK
Crystal structure of Ccp1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 86.814, 86.814, 158.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.824 - 2.103 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22520 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36042 | |
| <I/σ(I)> | 23.86 | |
| Completeness [%] | 99.7 | |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 289 | 100mM C3H2Na2O4, 200mM NH4NO3, 18% PEG 3350, 15% EG |
