5GPD

Crystal structure of the binding domain of SREBP from fission yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	96.903, 96.903, 151.595
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.973 - 3.501
R-factor	0.2661
Rwork	0.265
R-free	0.27850
RMSD bond length	0.008
RMSD bond angle	1.090
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.060	0.497
Number of reflections	10643
<I/σ(I)>	18.5	2.9
Completeness [%]	98.1	99.8
Redundancy	3.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M BTP (pH 7.0), 1.5M NaNO3