5GP4
Lactobacillus brevis CGMCC 1306 Glutamate decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 82 |
| Detector technology | CCD |
| Collection date | 2016-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97851 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.333, 170.129, 164.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.543 - 2.160 |
| R-factor | 0.18911 |
| Rwork | 0.187 |
| R-free | 0.23005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xey |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.983 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.176 | 0.700 |
| Number of reflections | 85974 | |
| <I/σ(I)> | 10.88 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.930 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 287 | 10% W/V PEG 2000 MME, 100mM Sodium Acetate/Acetic acid, 200mM Ammonium Sulfate |
