5GOR
Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.171, 179.282, 96.362 |
| Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
| Resolution | 30.497 - 2.673 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5goo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.976 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 84349 | |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 95.4 | 97 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 27% PEG 6k, 0.1 M Becine |
