5GOB

Lys6-linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	24.730, 26.380, 45.120
Unit cell angles	98.07, 93.85, 106.54

Refinement procedure

Resolution	44.388 - 1.153
R-factor	0.1931
Rwork	0.191
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.005
RMSD bond angle	1.014
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	1.200
High resolution limit [Å]	1.153	1.160
Rmerge	0.066	0.749
Number of reflections	23485
<I/σ(I)>	11.25
Completeness [%]	62.0
Redundancy	3.54	3.09

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	289	0.2M Magnesium chloride hexahydrate, 20% PEG 3350, PH5.9