5GNX
The E171Q mutant structure of Bgl6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.247, 95.697, 94.976 |
| Unit cell angles | 90.00, 125.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.12419 |
| Rwork | 0.123 |
| R-free | 0.15117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oin |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.442 |
| Data reduction software | PHENIX |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.081 |
| Number of reflections | 84462 | |
| <I/σ(I)> | 48.3 | 25.8 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.990 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25% PEG 8K, 600 mM NaCl, 100 mM Tris-HCl pH 8.0, 1M glucose |
