5GNV

Structure of PSD-95/MAP1A complex reveals unique target recognition mode of MAGUK GK domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97928
Spacegroup name	C 2 2 21
Unit cell lengths	62.445, 156.861, 59.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.089 - 2.596
R-factor	0.2171
Rwork	0.215
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	9354
<I/σ(I)>	31.8
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	289	0.2M lithium sulfate, 0.1M Bis-Tris pH 6.5 and 25%(w/v) polyethylene glycol 3350