5GNR

the structure of mini-MFN1 K88A in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	71.340, 74.975, 94.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.959 - 2.650
R-factor	0.2248
Rwork	0.216
R-free	0.30160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.522
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.650
Number of reflections	15269
<I/σ(I)>	21.38
Completeness [%]	99.8
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	200 mM ammonium fluoride, 20% (w/v) PEG 3350