5GNF
Crystal structure of anti-CRISPR protein AcrF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.944, 84.944, 63.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.472 - 1.500 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19190 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 0.403 |
| Number of reflections | 41528 | |
| <I/σ(I)> | 43.8 | 3.4 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 15.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 20% (w/v) PEG 8000, 100mM MES, 200mM Calcium acetate |
