5GN3
Structure of selenomethionine-labelled Uracil DNA glycosylase (BdiUNG) from Bradyrhizobium diazoefficiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-07-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.956, 90.068, 256.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.990 - 2.281 |
R-factor | 0.1808 |
Rwork | 0.180 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.193 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.990 | 2.320 |
High resolution limit [Å] | 2.281 | 2.281 |
Number of reflections | 132492 | |
<I/σ(I)> | 21.39 | |
Completeness [%] | 91.7 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 20% PEG3350, 200 mM sodium citrate, 100 mM sodium citrate/citric acid |