5GN0
Structure of TAZ-TEAD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.660, 120.900, 196.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2034 |
| Rwork | 0.200 |
| R-free | 0.26013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jua |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.734 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 42312 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 98.6 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 6% Polyethylene glycol 10000, 0.05M magnesium acetate, 0.1M tri-sodium citrate |
