5GMY

Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) tethered with an acceptor peptide containing the NVT sequon via a disulfide bond

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	121.549, 121.549, 181.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.100 - 3.500
R-factor	0.2282
Rwork	0.226
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wak
RMSD bond length	0.002
RMSD bond angle	0.561
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.106	1.283
Number of reflections	17649	828
<I/σ(I)>	44.7	3.6
Completeness [%]	99.8	100
Redundancy	13.9	14.2
CC(1/2)		0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10 mM MgCl2, 0.1 M Bis-Tris, pH 6.5, 22 % (w/v) PEG 550MME, 5% (v/v) Jeffamine M-600