5GMI
Crystal Structure of GRASP55 GRASP domain in complex with JAM-C C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 132.492, 132.492, 220.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.420 - 2.710 |
| R-factor | 0.259 |
| Rwork | 0.256 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.420 | 2.810 |
| High resolution limit [Å] | 2.710 | 2.711 |
| Rmerge | 0.137 | 0.667 |
| Number of reflections | 27006 | |
| <I/σ(I)> | 7.1 | 2.38 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | SODIUM FORMATE, SODIUM ACETATE |
