5GMH
Crystal structure of monkey TLR7 in complex with R848
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.099, 140.093, 151.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.200 |
| R-factor | 0.18669 |
| Rwork | 0.185 |
| R-free | 0.22483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gmf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.937 | |
| Number of reflections | 100054 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 98.8 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, ammonium sulfate, sodium citrate pH 5.2 |
