5GM8
Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruiginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.520, 66.610, 98.580 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 20.720 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cnd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.720 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.240 |
| Rmerge | 0.099 | 0.635 |
| Number of reflections | 34918 | |
| <I/σ(I)> | 8 | 1.9 |
| Completeness [%] | 95.5 | 95.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | Ammonium sulfate, Tris, Lithium sulfate |
