5GKJ
Structure of EndoMS in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 128.220, 70.120, 70.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.110 - 3.200 |
R-factor | 0.23 |
Rwork | 0.229 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5BSL |
RMSD bond length | 0.003 |
RMSD bond angle | 0.885 |
Data reduction software | MOSFLM |
Data scaling software | MOSFLM |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.920 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.073 | |
Number of reflections | 10762 | |
<I/σ(I)> | 17.7 | 10.1 |
Completeness [%] | 97.6 | 99.9 |
Redundancy | 6.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.16 M CALCIUM ACETATE, 80 MM SODIUM CACODYLATE, 14.4%(W/V) PEG8000, 20%(W/V) GLYCEROL |