5GKF
Structure of EndoMS-dsDNA1' complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.440, 92.440, 404.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.810 - 2.800 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5BSL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.041 | |
| Number of reflections | 24789 | |
| <I/σ(I)> | 19.1 | 5.4 |
| Completeness [%] | 93.9 | 95.6 |
| Redundancy | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.16 M CALCIUM ACETATE, 80 MM SODIUM CACODYLATE, 14.4%(W/V) PEG8000, 20%(W/V) GLYCEROL |
