5GK7
Structure of E.Coli fructose 1,6-bisphosphate aldolase bound to Tris
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.422, 72.671, 73.052 |
| Unit cell angles | 90.00, 103.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.16435 |
| Rwork | 0.163 |
| R-free | 0.19701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dos |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.062 | 0.488 |
| Number of reflections | 67264 | |
| <I/σ(I)> | 38.6 | 5 |
| Completeness [%] | 97.7 | 96.3 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 0.2 M ammonium acetate, 5mM fructose 1,6-bisphosphatase, 0.1 M Tris/HCl pH 7.0, and 15% PEG 4000 |
