5GJU

DEAD-box RNA helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	57.990, 57.990, 104.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.995 - 1.600
R-factor	0.1687
Rwork	0.167
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gxs
RMSD bond length	0.010
RMSD bond angle	1.158
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.221	28.995	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge		0.041	0.591
Rmeas	0.091	0.045	0.645
Rpim	0.036	0.018	0.255
Total number of observations	161038	5012	23561
Number of reflections	26166
<I/σ(I)>	12.5	28.2	3.4
Completeness [%]	99.8	96.1	100
Redundancy	6.2	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.1M DL-Malic acid, pH 7.0, 2mM AMP-PNP