5GJ4
Structure of NS2B-NS3 Protease from Zika Virus caught after self-cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0004 |
| Spacegroup name | P 31 |
| Unit cell lengths | 88.790, 88.790, 138.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.496 - 1.839 |
| R-factor | 0.1812 |
| Rwork | 0.169 |
| R-free | 0.19530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fp7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.736 |
| Data scaling software | Aimless |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.900 | 1.900 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.058 | 0.525 |
| Number of reflections | 105911 | |
| <I/σ(I)> | 14.1 | 1.51 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Sodium Malonate, pH 4.0, 10% PEG 3350 |
