5GIX
Human serum albumin-Palmitic acid-Fe(Hn3piT)Cl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-04-01 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.472, 94.550, 96.105 |
| Unit cell angles | 104.90, 89.90, 100.86 |
Refinement procedure
| Resolution | 27.126 - 2.801 |
| R-factor | 0.2133 |
| Rwork | 0.209 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.668 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 30492 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 96.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 293 | PEG3350, 50 mM potassium phosphate (pH 7.5), 5% glycerol, and 4% DMSO. |
