5GHL
Crystal structure Analysis of the starch-binding domain of glucoamylase from Aspergillus niger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 4 |
| Unit cell lengths | 75.360, 75.360, 91.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.980 - 2.000 |
| R-factor | 0.1809 |
| Rwork | 0.178 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nyi |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.157 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.107 | 0.077 | 0.164 |
| Total number of observations | 291560 | ||
| Number of reflections | 34419 | ||
| <I/σ(I)> | 44.5 | ||
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 8.5 | 7.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium sulfate, PEG400, Glycerol |
